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rb1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rb1
    Rb1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rb1/pm41617713-318-17-20
    Average 86 stars, based on 1 article reviews
    rb1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: GPX4-dependent ferroptosis sensitivity is a fitness trade-off for cell enlargement
    Article Snippet: anti-RB1 , Cell Signaling Technology , 9309; RRID: AB_823629.

    Article Title: BH3 mimetics targeting BCL-XL have efficacy in solid tumors with RB1 loss and replication stress
    Article Snippet: Cells were lysed in RIPA buffer (#PI89900, Fisher Scientific) supplemented with protease inhibitor (#PI78437, Fisher Scientific) and phosphatase inhibitor cocktails (#PI78426, Fisher Scientific).

    Knockdown:

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum- free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti- RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti- P53 (Santa Cruz, clone DO- 1, Cat# Sc- 126), anti- MYC (Cell Signaling, clone D84C12, Cat# 5605), anti- HA (Cell Signaling, clone 6E2, Cat# 2367), and anti- GAPDH Chen, Gardner et al. eLife 2024;13:RP93170. .. DOI: https://doi.org/10.7554/eLife.93170 14 of 20 (Cell Signaling, clone 14C10, Cat# 2118).

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum-free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti-RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti-P53 (Santa Cruz, clone DO-1, Cat# Sc-126), anti-MYC (Cell Signaling, clone D84C12, Cat# 5605), anti-HA (Cell Signaling, clone 6E2, Cat# 2367), and anti-GAPDH (Cell Signaling, clone 14C10, Cat# 2118). ..

    Western Blot:

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum- free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti- RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti- P53 (Santa Cruz, clone DO- 1, Cat# Sc- 126), anti- MYC (Cell Signaling, clone D84C12, Cat# 5605), anti- HA (Cell Signaling, clone 6E2, Cat# 2367), and anti- GAPDH Chen, Gardner et al. eLife 2024;13:RP93170. .. DOI: https://doi.org/10.7554/eLife.93170 14 of 20 (Cell Signaling, clone 14C10, Cat# 2118).

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum-free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti-RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti-P53 (Santa Cruz, clone DO-1, Cat# Sc-126), anti-MYC (Cell Signaling, clone D84C12, Cat# 5605), anti-HA (Cell Signaling, clone 6E2, Cat# 2367), and anti-GAPDH (Cell Signaling, clone 14C10, Cat# 2118). ..

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: Cell line (mouse) , Mouse embryonic fibroblasts (MEF) , Global Stem , GSC-6001G , –. .. Antibody , Anti-RB1 (Clone 4H1) , Cell Signaling , Cat# 9309 , 1:500 (WB). .. Antibody , Anti-P53 (Clone DO-1) , Santa Cruz Biotechnology , Cat# Sc-126 , 1:500 (WB).

    Article Title: BH3 mimetics targeting BCL-XL have efficacy in solid tumors with RB1 loss and replication stress.
    Article Snippet: The siRNA targeting BIRC5 were from Cell Signaling Technologies (SignalSilence Survivin siRNA II #6546). .. The following primary antibodies were used for immunoblotting at 1:1000 dilution unless otherwise indicated: anti-RPA32/RPA2 (phospho S33, Abcam #ab211877, 1:2000), anti-H2A.X phospho Ser139 (clone 20E3, Cell Signaling Technology #9718), anti-p53 (Cell Signaling Technology #9282), anti-p21 (clone EPR3993, Abcam #ab109199), anti-Survivin (Cell Signaling Technology #2808), anti-RB1 (clone 4H1, Cell Signaling Technology #9309, 1:2000), anti-Bad (#9239, Cell Signaling Technology, 1:500), anti-BAK (#12105, Cell Signaling Technology), anti-BAX (#5023, Cell Signaling Technology), anti-β-actin (#ab6276, Abcam), anti-BCL2 (#4223, Cell Signaling Technology, 1:500), anti-BCL-XL (#2764, Cell Signaling Technology), anti-BIM (#2933, Cell Signaling Technology), anti-cleaved caspase 3 (#9664, Cell Signaling Technology1:250), anti-MARCH5 (#06-1036, EMD Millipore, 1:500), anti-MCL1 (#5453, Cell Signaling Technology), anti-NOXA (#ab13654, Abcam, 1:250), anti-PARP (#9532, Cell Signaling Technology), anti-phosphoeIF2α Ser51 (#9721, Cell Signaling Technology), anti-PUMA (#12450, Cell Signaling Technology, 1:500), or anti-vinculin (#sc-73614, Santa Cruz Biotechnology, 1:20,000). .. The secondary antibodies were 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary (Promega).

    Selection:

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum- free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti- RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti- P53 (Santa Cruz, clone DO- 1, Cat# Sc- 126), anti- MYC (Cell Signaling, clone D84C12, Cat# 5605), anti- HA (Cell Signaling, clone 6E2, Cat# 2367), and anti- GAPDH Chen, Gardner et al. eLife 2024;13:RP93170. .. DOI: https://doi.org/10.7554/eLife.93170 14 of 20 (Cell Signaling, clone 14C10, Cat# 2118).

    Article Title: Metastatic small cell lung cancer arises from TP53/RB1-deficient and MYC overproduction hESC-derived PNECs
    Article Snippet: High titer viral particles were used to transduce hESCs in serum-free conditions, and antibiotic selection of transduced hESCs was performed without MEF feeder cells, using mTeSR1 stem cell media (STEMCELL Technologies). .. The efficiency of RB1 or P53 knockdown and production of MYC or MYC (T58A) was determined by western blot after antibiotic selection using the following antibodies: anti-RB1 (Cell Signaling, clone 4H1, Cat# 9309), anti-P53 (Santa Cruz, clone DO-1, Cat# Sc-126), anti-MYC (Cell Signaling, clone D84C12, Cat# 5605), anti-HA (Cell Signaling, clone 6E2, Cat# 2367), and anti-GAPDH (Cell Signaling, clone 14C10, Cat# 2118). ..

    Incubation:

    Article Title: Enhanced innate responses in microglia derived from retinoblastoma patient-specific iPSCs.
    Article Snippet: Correspondence Zi-Bing Jin, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730 China.. Email: jinzibing@foxmail.com Abstract RB1 deficiency leads to retinoblastoma (Rb), the most prevalent intraocular malignancy.. Tumor-associated macrophages (TAMs) are related to local inflammation disorder, particularly by increasing cytokines and immune escape.



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